摘要
目的探讨低分子量姬松茸多糖(LMPAB)对肿瘤细胞与内皮细胞迁移和黏附的影响。方法采用MTT法检测LMPAB对人结肠癌细胞(HT-29)和HUVECs增生的影响。采用分光光度法检测LMPAB对HT-29细胞与HUVECs黏附的影响,采用transwell双层小室观察LPMPAB对HT-29迁移穿过单层HUVECs的影响。结果LMPAB对HT-29和HUVECs增生无显著性作用,LMPAB能够降低HT-29细胞与内皮细胞的黏附,并减少迁移和穿过单层内皮细胞的肿瘤细胞数目。结论低分子量姬松茸多糖通过抑制肿瘤细胞与内皮细胞的黏附以及肿瘤细胞迁移穿过内皮细胞而发挥其抗肿瘤转移作用。
Objective To investigate the effects of low molecular weight polysaccharide lsolated from agaricus blazei murill （LMPAB） on adhesion and migration of tumor cell and HUVECs. Methods HT- 29 cell and HUVECs proliferation was analyzed by MTT. Adhesion and transmigration monolayer HUVECs ability of HT - 29 was examined by spectrophotometry and transwell chambers. Results LMPAB showed no eytotoxic effect on HT - 29 cells and HUVECs. LMPAB could significantly decrease HT - 29 adhesion and transmigration through HUVECs. Conclusion LMPAB exerts the anti - tumor metastasis activity via inhibition of tumor cell adhesion and migration HUVECs.
出处
《医学研究杂志》
2009年第7期32-34,135,共4页
Journal of Medical Research
基金
国家自然科学基金资助项目(30772751)
关键词
低分子量姬松茸多糖
黏附
肿瘤转移
迁移
Low molecular weight polysaccharide
Adhesion
Metastasis
Transmigration
