GC/MS同时检测人体尿液中可的宁和苯乙醇酸及苯乙醛酸的含量

Determination of cotinine,phenylglyoxylic acid and mandelic acid in human urine by GC/MS

目的：建立一种同时检测人尿样中可的宁（COT）、苯乙醛酸（PA）和苯乙醇酸（MA）含量的GC/MS分析方法。方法：采用GC/MS法,EI检测器,色谱柱为DB-5MS毛细管柱。进样前尿样先经过氯仿萃取,吹干后用MSTFA衍生化。结果：COT在0.0002-3.5μg.ml^-1呈线性关系,PA和MA在1.25-160μg.ml^-1呈线性关系,定量限分别为0.0002μg.ml^-1、1.25μg.ml^-1和1.25μg.ml^-1,回收率分别为89.53%-102.4%、84.88%-91.46%和83.46%-113.6%。结论：本实验室合并了尼古丁和苯乙烯这两种环境污染物的检测方法,而且定量下限与单个检测方法相当,可用于同时监控和测定尼古丁和苯乙烯对人体的危害。

Objective： To establish a GC/MS method for analysis of cotinine （COT）, phenylglyoxylic acid （PA） and mandelic acid （MA） in human urine. Methods： Human urine samples were extracted by CC13and derivatized with MSTFA after dried completely. The contents of COT,PA and MA were measured by GC/MS method with DB-SMS capillary column and EI ion-source. Results： The calibration curves for COT in urine samples were linear over the concentration ranges of 0. 0002-3.5 μg.ml^-1,while PA and MA were both of 1. 254160 μg.ml^-1. The limits of quantification were 0.0002 μg.ml^-1,1.25 μg.ml^-1and 1.25 μg.ml^-1for COT,PA and MA,respectively. The assay recoveries for COT,PA and MA ranged from 89.53%-02.4% ,84.88%-91.46% method can detect cotinine,ph be used human in routine assessment body. and 83.46%-113.6% ,respectively. Conclusion： The established enylglyoxylic acid and mandelie acid simultaneously,which would and monitoring of the internal exposure to nicotine and styrene in human body.