国产新型钛镍合金室间隔缺损封堵器的组织相容性研究

Study on Histocompatibility of Domestic New Type Nitinol Alloy Ventricular Septal Defect Occluder

目的观察国产新型钛镍合金室间隔缺损(VSD)封堵器的组织相容性,并为其临床应用的可行性提供依据。方法选用健康犬20只,在静脉复合麻醉、气管插管、动物呼吸机辅助呼吸下,植入国产新型钛镍合金VSD封堵器。植入后待其清醒送回动物房饲养。术后口服抗生素,饲养一段时间后处死,按动物处死时间(1、2、3、6个月)不同分为4组,每组5只,分别处死后对其行大体解剖、光镜、电镜检查观察封堵器位置、内膜覆盖情况及炎性细胞变化。应用SPSS 11.0软件进行统计学分析。结果光镜观察显示术后随时间延长,犬心内膜厚度不断增加,术后不同时间点差异显著(P<0.05)。术后1～3个月同一时间点左右心室内膜厚度无明显不同。术后随时间延长封堵器表面炎性细胞数逐渐减少(P<0.05)。电镜观察显示1个月封堵器开始出现明显的内皮细胞和少量纤维细胞,随时间延长,封堵器上逐渐长满内皮细胞,6个月时形成内皮组织。结论国产新型钛镍合金VSD封堵器具有良好的组织相容性,可以适应临床需要。

Objective To study the histocompatibility of domestic new type nitinol alloy ventricular septal defect（VSD） occluder in order to test the feasibility for clinical application. Methods The domestic new type nitinol alloy VSD occluder was inserted into 20 healthy dogs with intravenous balanced anesthesia and tracheal intubation, assisted by animal breathing machine respiration. The dogs were given oral antibiotic and would be killed in several months after operation. According to different slaughter time （ 1,2,3,6 months）, they were divided into 4 sections and including 5 dogs in each section. The occluder sites,the intimal coverage and the change of inflammatory cells were observed through gross anatomy,with the help of the light microscope and electron microscope. SPSS 11.0 software was used to analyze the data. Results The thickness of the endocardial enhanced with the time （ P 〈 0.05 ）. The thickness of the endocardial was not different at the same time between the left ventricle and the right ventricle in the months after operation. The inflammatory cells reduced with the time（P 〈0. 05）. The endodermis cells on the eccluder were observed with electron microscope and these cells would grow into the endodermis after 6 months. Conclusions The histocomnatibilitv of domestic new type nitinol alloy VSD occluder is very well can adapt to clinical requirement.