摘要
采用砂培和水培的方法,以导入AtCIPK23基因的K326品种、未转化的K326以及常规品种NC89三个基因型烟草为材料,研究了转AtCIPK23基因烟草品系的干物质和钾积累特征,以及根系生理特性差异。结果表明,在低供钾水平下,转AtCIPK23基因烟草的干物质积累以及各器官钾含量高于未转化的K326和NC89,并且具有较强的根系活力。钾吸收动力学研究结果表明,转AtCIPK23基因烟草的米氏常数(KM)和最低浓度(Cmin)低于未转化的K326和NC89,而不同基因型烟草间最大吸收效率(Vmax)无明显差异。
The method of sand culture and water culture were adopted to investigate the characteristics of dry matter and potassium accumulation and the physiological differences in the roots among AtCIPK23-transgenic K326, wild-type K326 and NC89. The results showed that the transgenic strain displayed higher dry matter accumulation, organ potassium content and a stronger root vitality than the wild strains under the condition of low potassium supplying. The study on K absorption kinetics indicated that the transgenic strain had lower KM and Cmin than the wild stains did. There were no significant differences in Vmax among the three strains.
出处
《河南农业科学》
CSCD
北大核心
2009年第6期53-56,共4页
Journal of Henan Agricultural Sciences
基金
国家烟草专卖局科技攻关项目(19980664)
关键词
转AtCIPK23基因烟草
钾累积
干物质
根系活力
AtCIPK23-transgenic tobacco strain
Potassium accumulation
Dry matter accumula tion
Root vitality
