盐酸克伦特罗在商品肉鸡血清中残留消除规律的研究

Study on the Residues and Elimination Pattern of Clenbuterol in Commercial Broiler Serum

为了研究肉鸡血清中盐酸克伦特罗残留消除规律,将100只商品肉鸡随机分为2组,从29日龄开始饲喂添加2种不同浓度的盐酸克伦特罗的饲料,采集不同时期的血液,分离血清,用高效液相色谱法(HPLC)进行测定。结果表明:盐酸克伦特罗在商品肉鸡体内吸收较快,饲喂第1天即可从血清中检出,用药期有2个药物残留高峰,分别是给药后的第5天和第21天;且随着饲喂剂量的增加,血清中药物的残留浓度增大。停药期盐酸克伦特罗在血清中残留浓度逐渐降低,前3 d降低较快,随着饲喂剂量的增加,消除的时间延长。

To investigate the residues and elimination pattern of clenbuterol in commercial broiler, 100 commercial broilers were randomly divided into two groups and feed with 1 or 3 mg/kg clenbuterol dietary,starting from 29 day-age. The serum was sampled at different time points and the residues of clenbuterol were analyzed by the method of high performance liquid chromatography （HPLC）. The results showed that the clenbuterol was absorbed quickly by broilers,which can be detected in serum at the first day post treatment and the quantity of clenbuterol reached peak at 5th and 21th day. The residues of clenbuterol in broilers feed with 3 mg/kg dietary were much higher than those feed with 1 mg/kg dietary. The residues concentration of clenbuterol in serum decreased rapidly in the first 3 days and then decreased gradually during the subsequent withdrawal time.