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急性胰腺炎胰腺腺泡细胞凋亡对大鼠腹腔巨噬细胞功能影响的研究 被引量：1

Effect of Apoptosis of Pancreatic Acinar Cells in Acute Pancreatitis on Intraperitoneal Macrophage Activation in Rats
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摘要 目的:研究急性胰腺炎胰腺腺泡细胞凋亡对巨噬细胞免疫功能的影响。方法:采用奥曲肽(OCT)诱导cae-rulein处理的AR42J细胞凋亡作用于大鼠腹腔巨噬细胞,检测巨噬细胞的粘附和吞噬功能;使用ELISA试剂盒检测细胞培养上清中TNF-α的含量。结果:急性胰腺炎胰腺腺泡细胞凋亡显著降低巨噬细胞的粘附功能和吞噬功能(P<0.05),同时能降低巨噬细胞分泌TNF-α的功能。结论:急性胰腺炎胰腺腺泡细胞凋亡能降低巨噬细胞的功能。 Objective： To investigate the effect of apoptosis of pancreatic acinar cells in acute pancreatitis on the im- mune function of macrophages. Methods： The AR42J cells were treated with octreotide and caerulein. Peritoneal macro- phages obtained from rats were stimulated with the AR42J cells. The adherence, and phagocytic capacity of the macro- phages were evaluated. The TNF-α content in the culture supernatant was determined by ELISA. Results..Apoptosis of pancreatic acinar cells in acute pancreatitis significantly depressed adherence and phagocytic ability of macrophages （P〈 0.05）, and reduced the secretion of TNF-α by the macrophages （P〈0.05）. Conclusion： Apoptosis of pancreatic acinar cells in acute pancreatitis could decrease the immune function of macrophages.
作者 梁桃 马占军 尚国印 史立军
机构地区 哈尔滨医科大学第一临床医学院
出处 《中国康复》 2009年第3期153-155,共3页 Chinese Journal of Rehabilitation
关键词 急性胰腺炎 胰腺腺泡细胞 凋亡 巨噬细胞 Pancreatitis Pancreatic acinar cells： Apoptosis Macrophage
分类号 R576 [医药卫生—消化系统]
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参考文献7

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二级参考文献6

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共引文献9

同被引文献14

  • 1胡梅洁,曾晖,章永平,张曙,乔敏敏,付华,吴云林.苦参碱联合5-氟尿嘧啶对人胃癌裸鼠移植瘤的抑制作用[J].中华消化杂志,2005,25(4):219-221. 被引量：27
  • 2田雄英,翁山耕,林永堃,程协枝,林丽娟.苦参碱对大鼠肝星状细胞增殖和凋亡的影响[J].肝脏,2005,10(2):95-97. 被引量：29
  • 3金艳书,吴学敏,娄金丽.苦参碱对人肝癌细胞增殖、细胞周期及细胞凋亡的影响[J].中国临床康复,2006,10(3):107-109. 被引量：38
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  • 7Schuld A,Birkmann S,Beitinger P,et a1.Low doses of dexamethasone affect immune parameters in the absence of immunological stimulation[J].Exp Clin Endocrinol Diabetes,2006,114(6):322-328.
  • 8He Z J,Podkletnova I,Alho H,et al.Apoptosis in acute pancreatitis[J].Ann Chir Gynaecol,2000,89:65-67.
  • 9Matsuzaki I,Suzuki H,Kitamura M,et al.Cisplatin induces fas expression in esophageal cancer cell lines and enhanced cytotoxicity in combination with LAK cells[J].Oncology,2000,59(4):336-343.
  • 10Kaiser A M,Saluja A K,Sengupta A,et al.Relationship betweenseverity,necrosis,and apoptosis in five models of experimental acutepancreatitis[J].Am J Physiol,1995,269(5):1295-1304.

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中国康复
2009年 第3期

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