乙醇脱氢酶(ADH)家族生物信息学分析

Analysis of Alcohol Dehydrogenase(ADH) Family by Bioinformatics

本研究用生物信息学的方法分析了玉米等7个物种ADH的保守功能域、蛋白二级结构和进化关系。分析证实,植物ADH通常含有3个保守功能域,它们具有GroES结构的ADH_N结构域,Rossmann折叠NAD(P)(+)结合蛋白和锌结合位点,这3个保守结构在物种中具有相当的保守性。二级结构的分析表明,在我们研究的物种中,每个物种的两个ADH同工酶折叠情况大体相同,物种间蛋白二级结构也趋向一致。通过构建系统进化树,分析了供试物种中ADH以及ADH两个同工酶间的进化关系。初步显示,在进化上,单、双子叶植物源自不同的ADH祖先。双子叶植物ADH在物种间具有差异;而在单子叶植物不同物种其ADH同工酶出现分化。

We analyzed the conservative functional domains, protein secondary structure and their evolutionary relationships among alcohol dehydrogenase （ADH） of maize and other six species by using approaches ofbioinformatics. The results comfirmed that the plant ADHs generally contain three functional domains, which commonly are ADH_N domain of GroES structure, NAD（P）（＋）-binding protein by Rossmann fold and zinc banding site, these functional domains would be considered much conservative in all of plant species. Analysis of secondary structure showed that the folded form of two ADH isozymes are quite similar in tested species, and the protein secondary structure of all species tended to be more consistent. By constructing phylogenectic tree and analyzing the evolutionary relationships between two ADH isozymes in tested species, the results indicated that ADH of Momocotyledon plant and dicotyledon plant should not be derived from the same ancestors. ADH in dicotyledon exists diversity in the species, as well as the ADH isozyme in different monocotyledon species appears obviously differentiation.