番茄抗黄化曲叶病毒育种研究进展

Progress of Breeding for the Control of Tomato Yellow Leaf Curl Virus in Tomato

本文分别对近年番茄抗黄化曲叶病毒的传统育种、分子辅助育种、基因工程育种进展进行了综述。栽培番茄均不抗番茄黄化曲叶病毒,所以传统育种采用从野生近缘种中筛选抗性材料,以其为亲本与栽培番茄进行杂交来获得抗性;野生近缘种中的抗性位点Ty-1、Ty-2和Ty-3及一些QTLs先后被定位,也筛选出了可鉴定Ty-1基因的SSR-47标记及鉴定Ty-3的SCAR标记;通过转基因技术获得抗性是研究热点之一,目前转入番茄后表现出抗性的序列有TYLCV病毒的CP基因、REP基因的部分序列或反义序列、不编码的保守序列以及源于白粉虱的GroEL基因。同时讨论了今后的主要发展方向。

This paper has reviewed the recent research progress in tomato of enhancing the resistances to tomato yellow leaf curl virus （TYLCV）, including filtering, crossing, molecular assistance selection and transgenic methods. No resistance has been found to date in the Solarium lycopersicum germplasm, sources of resistance to TYL-CV in different wild tomato species have been discovered, partial resistance to TYLCV was introduced from wild tomato species to tomato. Genetic studies on TYLCV resistance have revealed various sources of resistance in wild species, there were Ty-1, Ty-2, Ty-3 and some QTLs, the marker SSR-47 which can identify the resistance to TYLCV of Ty-1 has been selected, SCAR marker closely linked to Ty-3 was designed also. Resistance derived through genetic engineering has many advantages. There are a number of reports to develop genetically engineered resistance to TYLCV through the introduction of partial and full-length of CP gene, REP gene or non-coding sequences, as well as GroEL gene. The prospects of tomato breeding for control of TYLCV have also been discussed.