高效液相色谱法同时测定参橘止咳滴丸中肉桂酸、橙皮苷和哈巴俄苷含量

Simultaneous determination of cinnamic acid,aurantriamarin and harpagoside in Shenju Zhike drop pill by high performance liquid chromatography

建立了用高效液相色谱法同时测定参橘止咳滴丸中肉桂酸、橙皮苷和哈巴俄苷含量的方法，色谱柱为Hypersil C18，流动相为甲醇-0．2％醋酸溶液，梯度洗脱，检测波长280nm，柱温25℃．结果显示，肉桂酸、橙皮苷和哈巴俄苷检测线性范围分别为0．00814—0．20350，0．00968～0．24200和0．00684～0．10260mg·mL^-1，平均回收率为98％-100％，相对标准偏差小于2．19％．方法简便、快速、准确，可用于参橘止咳滴丸的质量控制．

It was aimed to establish an HPLC method for simultaneous determination of three kinds of components in Shenju Zhike drop pill i.e. cinnamic acid, aurantriamarin and harpagoside. Samples were analyzed by gradient elution on Hypersil-C18 column, MeOH-0.2% acetic acid was the mobile phase, the detection wavelength was 280 nm and the temperature of column was 25℃. The results showed that the linear ranges of cinnamic acid, aurantriamarin and harpagoside were 0. 008 14 - 0. 203 50, 0. 009 68-0. 242 00, 0.006 84 -0. 102 60 mg.mL^-1, respectively. The average recovery rate was 98% - 100% , RSD 〈 2.19%. Therefore, the simple, rapid and accurate method could be used for the quality control of Shenju Zhike drop pill.