蝇蛆壳聚糖黏均分子量的测定

Determination of the Viscosity-average Molecular Weight of Fly Maggot Chitosan

目的:测定蝇蛆壳聚糖特性黏度,并计算其黏均分子量。方法:利用乌式黏度计,测定高聚物蝇蛆壳聚糖的黏度,采用外推法和一点法计算,进而得出其分子量。结果:外推法和一点法均可用于壳聚糖黏度及分子量的测定。结论:一点法只需测得1个浓度下的壳聚糖溶液的相对黏度和增比黏度,即可简便、快速、准确地测出壳聚糖的特性黏度,进而求得其黏均分子量,相对一步法更简便,但外推法的可靠性及准确性更高。

Objective： To determine the viscosity-average molecular weight of fly maggot chitosan. Method： Ubbelohde viscometer was used to measure the viscosity of fly maggot chitosan solution, and extrapolation and one-point methods were adopted to calculate the relative molecular weight of the chitosan. Result： The viscosity and molecular weight of chitosan were obtained with both extrapolation method and one point method. Conclusion： One point method is convenient, fast and accurate for the determination of viscosity and molecular weight of chitosan, while extrapolation method is even more accurate.