不同参数对超声辐照下细胞膜通透性影响的研究

Effects of Different Parameters on Hepatocyte Membrane Permeability under Microbubble Enhanced Ultrasound Exposure

目的:探讨不同参数条件下超声造影剂对正常肝细胞膜通透性的影响。方法:将用于诊断的超声辐照含全氟显微泡的HL-7702细胞悬液,频率2MHz,设置不同的辐照时间、机械指数、微泡浓度。辐照后观察大分子荧光物质进入细胞情况以检测"声致孔隙"作用。结果:随着辐照时间的延长,MI的升高,微泡浓度的增加,"声致孔隙"作用均有增加。结论:用于诊断的超声结合全氟显可诱导HL-7702细胞悬液发生"声致孔隙"作用,辐照时间、机械指数、微泡浓度是其重要影响因素。

Objeetive：To investigate the effects of different parameters on hepatocyte membrane permeability under microbubble enhanced ultrasound exposure. Methods：Suspensions of HL-7702 were exposed to diagnostic ultrasound at fre quency of 2MHz,with different exposure time,mechanical index （MI） and microbubble concentration. The uptake of fluo rescein isothiocyanatedextran （FD500） by HL 7702 was observed,and the percentages of sonoporation cells were counted. Results：Sonoporation was increased with exposure time, MI and microbubble concentration. Conclusion： Sonoporation was observed in HL-7702 in the suspension with the addition of perfluoropropane albumin microsphere exposed by diagnostic ul trasound,and could be affected by exposure time, MI and microbubble concentration.