缺氧低糖培养对少突胶质前体细胞的影响

Influence of oxygen deprivation combined with low glucose on O2A progenitors in vitro

目的：探讨缺氧低糖环境对体外培养少突胶质前体细胞（O2A细胞）的影响。方法：应用台盼蓝染色计数细胞存活率，Hochest33258荧光染色及流式细胞仪分析细胞凋亡情况，透射电镜观察细胞形态变化。结果：缺氧培养3h细胞变化不明显，但缺氧培养6h，细胞存活率明显下降，凋亡细胞明显增多，并且随着缺氧时间延长存活细胞更少、凋亡细胞更多，至缺氧培养12h，大多数细胞破碎。结论：低糖联合缺氧培养模式可以建立可行的O2A细胞外缺氧模型。

Objective： To study the influence of oxygen and glucose deprivation on O2A progenitors. Methods： Trypan blue staining was used to measure O2A progenitors viability. Hochest 33258 fluorescent staining and flow cytometry techniques were used to measure apoptotic cells. Results： The apoptotic O2A cells were not found until the oxygen deprivation combined with low glucose were maintained for 6 h, and promoted in a time-dependent manner. Additional 6 h later, necrosis occured to most O2A cells. Conclusion ： Oxygen deprivation combined with low glucose can mimic the ischemic-hypoxic damage of O2A progenitors in vitro.