Peroxiredoxin Ⅲ在大鼠超负荷心肌肥厚过程中的表达变化

Change of peroxiredoxin Ⅲ mRNA expression in process of rats pressure-overload cardiac hypertrophy

目的：探讨Peroxiredoxin Ⅲ（PrxⅢ）在大鼠超负荷心肌肥厚过程中的表达变化。方法：雄性SD大鼠随机分为假手术对照组和模型组，模型组大鼠实施腹主动脉缩窄术，制备压力超负荷心肌肥厚模型。分别于术后24h，2、4、6周，测定血液动力学变化；称量心体质量；光镜和电子显微镜观察左心室的组织变化；提取左心室RNA，RT-PCR分析PrxⅢ的表达变化。结果：术后24h两组大鼠的血液动力学和心肌形态无明显变化。术后2、4、6周时，模型组大鼠的SBP、DBP、LVSP、LVED及左心室体质量指数逐渐增加，心肌纤维逐渐增粗，线粒体逐渐增多，肌节增长。术后24h PrxⅢ的表达稍有降低，但无统计学意义，2周时则明显降低，4周有所上升但仍低于对照，6周时明显上升且高于对照组。结论：PrxⅢ mRNA表达呈现先降低后增高的趋势，即在心肌肥厚中早期表达降低，而在心肌肥厚形成后表达明显升高，表明PrxⅢ参与了超负荷心肌肥厚的发生。

Objective： To investigate the expression change of peroxiredoxin Ⅲ（PrxⅢ） in process of rats overload cardiac hypertrophy. Methods： Male SD rats were divided randomly into a shame operation control group and a model group. Cardiac hypertrophy model was made through partial ligation of the abdominal aorta. After 24 h, 2 weeks, 4 weeks, 6 weeks of operation, physiological index was first detected, then left ventricle was observed under light and electron microscopes and PrxⅢ mRNA expression was measured. Results： Model of rat cardiac hypertrophy was formed on 6 weeks after operation through analyzing the change of the physiological index and morphology. Compared to the control group, the expression of PrxⅢ mRNA was lowered on 24 h and further lowered on 2 weeks. PrxⅢ mRNA began to enhance but was still lower than that of control on 4 weeks; On 6 weeks, PrxⅢ mRNA was significantly higher than that of the control group. Conclusion： The expression of PrxⅢ first decreased and then increased during the process of rat pressure-overload cardiac hypertrophy. PrxⅢ may take part in the forming and development of pressure-overload cardiac hypertrophy.