巢蛋白及神经元素3在胚胎早期发育中的表达与分布

Expression and distribution of nestin and neurogenin 3 in early embryonic development

目的：比较巢蛋白（Nestin）与神经元素3（Ngn3）在胚胎早期发育各组织中的表达与分布。方法：采用免疫组织化学SABC法对30例5-10周人胚胎发育中各器官组织中Nestin与Ngn3的表达与分布进行定位研究。结果：5周时，肝造血干细胞Nestin、Ngn3阳性，胰芽原始导管上皮细胞、皮肤上皮细胞也呈Ngn3阳性反应；8周后，胚胎皮肤、椎间盘及肾上腺组织Nestin阳性，而Ngn3表达为阴性。结论：Nestin、Ngn3在胚胎早期发育不同组织细胞的表达有不同的时空性，确切意义及机制有待进一步研究。

Objective： To compare expression and distribution of Nestin and Neurogenin （Ngn3） in embryonic tissue during the early development. Methods： Sample collection was conducted in accordance to the principle of informed consent. Immunohistochemical SABC method was adopted in studying at 5-10 weeks of gestation, and localization analysis was made on Nestin and Ngn3 expression for tissue in embryonic development. Results： At 5 weeks of gestation, hemopoietie stem cells of the liver presented Nestin and Ngn3-positive reaction and primitive ductal epithelium of pancreas and epithelium of the skin showed Ngn3-positive reaction. After 8 weeks of gestation, hepatic cells, skin, suprarenal gland and intervertebral discs all expressed Nestin-positive and Ngn3-negative. Conclusion： The regulatory roles of Nestin and Ngn3 on cellular differentiation in the early embryonic development have different “space-time”. Their certain significances and mechanisms remain further study.