HPLC法测定尼莫地平脂肪乳中主药的含量

Determination of Main Component in Nimodipine Emulsion by HPLC

目的：建立以高效液相色谱法测定尼莫地平脂肪乳中尼莫地平含量的方法。方法：色谱柱为Lichrospher C18，流动相为甲醇-水-四氢呋喃（38：29：29），流速为1．3mL·min^-1，检测波长为237nm，柱温为39℃，进样量为20μL。结果：尼莫地平检测浓度的线性范围为1．0～40．0μg·mL。（r=0．9998）；平均回收率为98．62％，RSD=1．28％。结论：本方法可用于该制剂的含量测定。

OBJECTIVE： To establish an HPLC method for the determination of the content of nimodipine in nimodipine emulsion. METHODS： The sample was separated on Lichrospher C18 column with methanol- water- tetrahydrofuran （38 ： 29 ： 29） as the mobile phase at a flow rate of 1.3 mL · min ^-1. The detection wavelength was set at 237 nm; The column temperature was set at 39 12 and the sample size was 20μL. RESULTS： The linear range of nimodipine was 1.0--40.0μg · mL^-1 （r = 0.999 8） and the average recovery rate was 98.62% （RSD = 1.28%） . CONCLUSION： The established HPLC method is applicable for the determination of nimodipine content in nimodipine emulsion.