阿司匹林对肠上皮细胞的损伤作用及机制

Effects and mechanism of aspirin on enterocyte injury

目的:探讨阿司匹林对肠上皮细胞增殖的影响及作用机制.方法:将不同浓度的阿司匹林溶液与肠上皮细胞株Caco-2细胞共同培养24h及48h,采用MTT法检测阿司匹林对Caco-2细胞的增殖作用.建立Caco-2单层细胞模型,采用不同浓度阿司匹林溶液处理后,用EVOM电压电阻仪测定细胞跨膜电阻抗(TER)变化.结果:在不同浓度的阿司匹林(0、0.1、1、10mmol/L)作用下,24h细胞存活率分别为96.67%±1.13%、84.32%±1.29%、62.33%±2.02%和42.99%±2.09%;48h细胞存活率分别为96.45%±1.21%、76.89±2.28%、50.28%±0.98%和32.66%±1.99%,阿司匹林对Caco-2细胞的增殖作用呈现剂量-时间依赖性(P<0.05).与对照组相比,阿司匹林作用组TER明显降低,至10mmol/L组作用72h时,TER值降低至50.1%.结论:阿司匹林可抑制肠上皮细胞的增殖,同时损伤细胞屏障功能,损伤紧密连接,且以上两种作用呈剂量和时间依赖性.

AIM： To investigate the effect and mechanism of aspirin on proliferation of enterocytes. METHODS： After co-culture of aspirin solution with Caco-2 cells for 24 h and 48 h, the proliferation of Caco-2 cells in each group was examined using MTT. Caco-2 monolayer cells model was established. After treatment with different concen- trations of aspirin, Transepithelial resistance （TER） of cells was measured by EVOM voltohmmeter. RESULTS： After 24 h, the cell survival rates were 96.67% ± 1.13%, 84.32% ± 1.29%, 62.33% ± 2.02% and 42.99% ± 2.09% in groups with aspirin of 0, 0.1, 1, 10 mmol/L, respectively; after 48 h, the cell survival rates were respectively 96.45% ± 1.21%, 76.89% ± 2.28%, 50.28% ± 0.98% and 32.66% ± 1.99%. The TER in the group with aspi- rin of 10 mmol/L was reduced to 50.1% after 72 h. Multiple factors chi square test showed that the influence of aspirin on the proliferation of Caco-2 cells and the epithelial barrier was dosedependent and time-dependent. CONCLUSION： Aspirin could inhibit the prolifer-ation of enterocyte and affect the epithelial barrier, in a dose-dependent and time-dependent manner.