核心蛋白聚糖在原发性肝癌中的表达

Expression of decorin in primary hepatocellular carcinoma

目的:研究核心蛋白聚糖(decorin,DCN)在原发性肝癌组织中的表达.方法:免疫组织化学(SP法)分别检测16例正常肝组织、20例肝硬化组织、30例肝癌及癌旁组织中DCN的表达,利用阳性表达率及吸光度进行半定量及定量分析.结果:DCN在肝硬化及癌旁组织中呈强阳性表达,阳性表达率、平均吸光度与正常肝组织及肝癌组织相比,有显著性差异(55%,73.3%vs0%,10%;0.2357±0.0396,0.2983±0.1990vs0.1394±0.0072,0.1589±0.0115,均P<0.01).结论:DCN可能作为一种负性调控蛋白参与肝癌及肝纤维化的发生发展.

AIM： To study the expression of decorin （DCN） in primary hepatocellular carcinoma （PHC） tissues. METHODS： Immunohistochemistry （SP） was used to detect DCN expression in 16 samples of normal hepatic tissues, 20 samples of liver cirrhosis tissues and 30 samples of PHC tissues and relative paracancerous tissues. The quantitative analysis was performed with positive expression rate and A value in this study RESULTS： Liver cirrhosis tissues and paracancerous tissues showed strong positive expres- sion of DCN. Their positive expression rates and the average A values had significant difference compared with normal hepatic tissues and PHC tissues （55%, 73.3% vs 0%, 10%; 0.2357 ± 0.0396, 0.2983 ± 0.1990 vs 0.1394 ±0.0072, 0.1589 ±0.0115, P 〈 0.01）. CONCLUSION： Our results indicate that DCN may pose negative effects on the development of cirrhosis and carcinoma in hepatic tissues.