NRP2表达抑制对结肠癌细胞LoVo移植瘤淋巴管生成和转移的影响

Influence of inhibition neuropilins-2 expression on lymphangiogenesis and metastasis in colorectal carcinoma

目的:探讨抑制Neuropilins-2(NRP2)基因表达对结肠癌淋巴管生成和转移的影响。方法:采用RNA干扰(RNAi)下调结肠癌LoVo细胞株NRP2表达,接种NRP2 RNAi的LoVo细胞株于裸鼠,建立裸鼠结肠癌结肠原位移植瘤模型,分别观察移植后4、6、8和12周裸鼠结肠原位移植瘤淋巴管生成以及转移的情况。结果:成功建立结肠癌裸鼠原位移植瘤动物模型。下调结肠癌LoVo细胞株NRP2表达能显著降低裸鼠结肠原位移植瘤的淋巴管密度以及远处转移。结论:抑制NRP2表达可以抑制结肠癌的淋巴管生成和转移,它将是一个潜在的肿瘤治疗靶点。

Objective：To explore the role and imquence of inhibition neuropilins-2 （NRP2） expression on lymphangiogenesis and metastasis in colorectal carcinoma. Methods：To downregulate NRP2 expression by RNA interference. To transfection NRP2 RNAi vector into LoVo cells and transplanted into nude mouses. After havesting the xenotransplant neoplasm respectively 4,6,8,12 weeks, the expression of NRP2 and proliferation, and the lymphantic density, lymphonode metastasis and distance organ metastasis in the xenotransplant tumor were dectected and compared with the control group. Results ： An orthotopic transplantation model of human primary colorectal cancer was successfully established in nude mice, which could completely repeat the natural clinicopathologic course of human primary colorectal cancer, and metastasis pattern of the model was similar to that of clinical patients. It significantly decreased the lymphantic density, lymphonode metastasis and distance organ metastasis in the xenotransplant tumor of LoVo cells, which downregulated NRP2 expression. Conclusion：To inhibite lymphangiogenesis and the metastasis in colorectal carcinoma by downregulating NRP2 expression, it may be a potential target of therapy for tumors.