正交试验法优选秦皮香豆素醇提工艺

Study on Optimum Process for Extracting Coumarin from Cortex fraxini by Orthogonal Test

研究了秦皮主要活性成分秦皮香豆素的最佳醇提工艺,并采用了毛细管区带电泳(CZE)快速分析了秦皮苷、秦皮甲素、秦皮乙素的含量。以3种秦皮香豆素含量作为考察指标,采用L9(34)正交表,通过考察乙醇体积分数、提取次数、提取时间和料液比4个因素对醇提效果的影响,得到秦皮最佳提取条件为:以体积分数65%的乙醇为提取溶剂,在料液比1∶9(g∶mL)的条件下,回流提取3次,每次1h,秦皮总香豆素的得率为3.61%。

The optimum process of extracting fraxin, esculin and esculetin as active coumarin from Cortex fraxini（ Qinpi ） bark was studied. A rapid and simple method used to determine fraxin, esculin and esculetin in C. fraxini by capillary zone electrophoresis （CZE） was described. The effects of EtOH volume fraction, extracting times, extracting time, and ratio of material to liquor on extraction yield of 3 kinds of coumarin were investigated by orthogonal test. The optimum extracting conditions confirmed by the index of extraction yield of 3 kinds of coumarin were as follows ： ratio of material to liquor 1 ： 9 （ g ： mL）, three times of reflux extraction with 65 % EtOH, each extraction 1 h. The yield of total coumarin was 3.61%