摘要
Asthenozoospermia (作为) 是人的男不孕的一个普通原因。在一研究，从不肥沃的人的超过 80% 样品减少了精子活动性。精液的血浆是从睾丸， epididymis 和几男附件腺的分泌物的混合物，包括前列腺，精囊和考珀的腺。研究证明了那精液的血浆包含为精子活动性是重要的蛋白质。为了推进，探索 pathophysiological 特性作为，我们用钠 dodecyl 硫酸盐 polyacrylamide 胶化电气泳动和在里面胶化消化把精液的血浆蛋白质与作为病人和健康施主分开了，然后使蛋白质遭到了到液体层析鈥搈a ss spectrometry (LC-MS/MS ) 分析。741 蛋白质的一个总数在精液的血浆被识别，与 3.3% 的假发现率。用光谱的数，我们发现 45 蛋白质是三方面的 upregulated， 56 蛋白质是在 AS 组的三方面的 downregulated 什么时候与控制相比。大多数这些蛋白质从 epididymis 和前列腺发源。这研究识别了男不孕的 biomarker 候选人的富有的来源并且显示 epididymis 和前列腺的功能的畸形能是贡献。我们识别了在其它地方被显示了涉及氧化应力(OS ) 的控制的 DJ-1 鈥攁 蛋白质鈥攁s 在同样精液的血浆的 downregulated 蛋白质。在同样精液的血浆的 DJ-1 的层次关于在控制样品的那些的一半。另外，反应的氧种类的层次更高是 3.3 褶层在同样样品比在控制。一起拿，这些数据建议 DJ-1 的 downregulation 在精液涉及 OS，因此影响精液的质量。
Asthenozoospermia （AS） is a common cause of human male infertility. In one study, more than 80% of the samples from infertile men had reduced sperm motility. Seminal plasma is a mixture of secretions from the testis, epididymis and several male accessory glands, including the prostate, seminal vesicles and Cowper＇s gland. Studies have shown that seminal plasma contains proteins that are important for sperm motility. To further explore the pathophysiological character of AS, we separated the seminal plasma proteins from AS patients and healthy donors using sodium dodecyl sulfate polyacrylamide gel electrophoresis and in-gel digestion, and then subjected the proteins to liquid chromatography-mass spectrometry （LC-MS/MS） analysis. A total of 741 proteins were identified in the seminal plasma, with a false discovery rate of 3.3%. Using spectral counting, we found that 45 proteins were threefold upregulated and 56 proteins were threefold downregulated in the AS group when compared with the control. Most of these proteins originated from the epididymis and prostate. This study identified a rich source of biomarker candidates for male infertility and indicates that functional abnormalities of the epididymis and prostate can contribute to AS. We identified D J-1--a protein that has been shown elsewhere to be involved in the control of oxidative stress （OS）-as a downregulated protein in AS seminal plasma. The levels of D J-1 in AS seminal plasma were about half of those in the control samples. In addition, the levels of reactive oxygen species were 3.3-fold higher in the AS samples than in the controls. Taken together, these data suggest that downregulation of DJ-1 is involved in OS in semen, and therefore affects the quality of the semen.
