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生物素标记的Tn抗原的合成 被引量：3

Synthesis of biotin-labeled Tn antigen
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摘要 以全乙酰化的2-叠氮基半乳糖基三氯乙酰亚胺酯为起始原料,通过与L-苏氨酸衍生物的糖基化反应以及叠氮还原,并同时进行N-乙酰化的一锅法反应制得全保护的Tn抗原,在脱去苏氨酸上的氨基保护基后,含生物素的琥珀酰亚胺酯与Tn抗原上的氨基发生选择性胺解得到标题化合物。目标产物及重要中间体的结构都通过1HNMR、13CNMR和MS进行了表征。 Through the glycosylation reaction between the fully acetylated 2-azido-galactosyltrichloroacetimidate and L-threonine derivative,followed by azido-teduction and N-acetylation in one pot the fully protected Tn antigen was obtained. After deprotection of amino group on threonine, amino-attached Tn antigen was lig- ated selectively with suecinimidyl ester containing biotin to afford the biotin-labeled tin antigen. The structures of desired product and key internaediates were characterized by ^1HNMR, ^13CNMR and MS.
作者 冯丽娟 孙志勇
机构地区 遵义医学院珠海校区生物工程系
出处 《化学试剂》 CAS CSCD 北大核心 2009年第5期334-336,361,共4页 Chemical Reagents
关键词 Tn抗原 生物素 糖基化反应 合成 Tn antigen botin glycosylation reaction synthesis
分类号 O621.3 [理学—有机化学]
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参考文献12

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同被引文献17

  • 1Beck ML, Red blood cell polyagglutination :clinical aspects[J]. Semin Hematol,2000,37(2) : 186-196.
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  • 9张春燕,刘杰,赵素珍.Tn多凝集红细胞致ABO疑难血型鉴定1例[J].临床输血与检验,2009,11(2):177-178. 被引量：5
  • 10王红梅,吕俊兰,吴沪生,陈春红.生物素代谢及其对基因表达影响的研究现状[J].中国实用儿科杂志,2009,24(11):885-887. 被引量：8

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化学试剂
2009年 第5期

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