摘要
运用ISSR技术对甜椒CMST6A及其相应的保持系T6B基因组DNA进行了比较分析,共使用了44条随机引物,其中33条引物在两系之中都获得了扩增产物。在不育系中获得了1条稳定扩增的特异片段ISSR-81400。通过随机抽取群体的不育和可育单株对ISSR引物进行鉴定,初步认为ISSR-81400可能是与甜椒CMS不育基因连锁的分子标记。
The comparative analysis of genomic DNAs between sweet pepper cytoplasmic male sterile line CMST6A and its maintainer line T6B was made by means of ISSR technique. Thirty-three primers amplified products in the two lines from 44 random primers, among which the specific fragment ISSR-81400of CMST6A was found. The primer ISSR-8 was identified with sterile plants and fertile plants. The result showed the marker ISSR-81400 is likely linked with male sterile gene.
出处
《江苏农业学报》
CSCD
北大核心
2009年第3期607-609,共3页
Jiangsu Journal of Agricultural Sciences
基金
江苏省科技基础设施建设计划项目(BM2008008)
江苏省农业科学院基金项目[6510711
CX(08)610]
关键词
甜椒
胞质雄性不育
ISSR标记
sweet pepper
cytoplasmic male sterility (CMS)
ISSR marker