大鼠神经元突触重塑参与神经免疫调节网络的功能重建

Reconstruction of neuron synapses is involved in the function reestablishment of rat neuroimmunoreglation network

目的探讨神经免疫调节网络的功能重建模式。方法用大鼠全基因芯片检测技术分析下丘脑外侧区神经免疫调节功能相关信号表达;用数据库分析差异基因的功能树。结果免疫组大鼠基因表达谱有632个差异基因(其中免疫2 d组374个,免疫4 d组62个,免疫6 d组196个);对差异基因中的398个上调差异基因进行功能树相关分析显示,27个基因为参与信号传导功能的已知功能基因,涉及31个细胞功能信号传导通路,涵盖了已知的突触重塑不同的信号传导通路。结论突触重塑可能构成神经免疫调节网络功能重建的重要模式。

Objective To reestablish neuroimmunoregulation network and its function. Methods Whole-some gene array was used to analyze the gene expression involved in neuroimmunoregulation function of the lateral hypothalamic （LH） in rats at different immunizational time. The function trees of different genes were analysed with the PathWay Miner public database. Results 632 genes were differentially expressed： including 374 of 2-day immunized group,62 of 4-day immunized group and 196 of 6-day immunized group. Function signal pathway analysis for 398 up-regulated genes showed 27 genes were involved in 31 cell functional signal conduction pathways, including the well-known signal conduction pathways of synaptic reconstruction. Conclusion Synapses reconstruction appears to be the important pattern of functional reestablishment of neuroimmunoregulation network.