特发性男性不育患者精浆蛋白质分析

Analysis of proteomics of seminal plasma from male patients with idiopathic infertility

目的寻找诊断特发性男性不育的生物标记分子。方法应用二维电泳与质谱技术(MS+MS/MS)分析特发性男性不育患者与正常对照组精浆蛋白质组成,寻找差异表达蛋白质。结果特发性男性不育患者较正常对照组精浆中血清白蛋白和前列腺酸性磷酸酶减少,而组织蛋白酶B和锌α2糖蛋白增加。结论特发性男性不育可能与精子获能受阻及精液免疫功能紊乱有关。

Objective To discover biological markers of male infertility. Methods Two-dimensional eleetrophoresis and Mass-Spectrum techniques （ MS ＋ MS/MS） were used for analyzing the seminal plasma from idiopathic male infertility and the control. Results Serum albumin and prostatic acid phosphatase were reduced in seminal plasma from idiopathic male infertility, while Cathepsin B and Zn-alpha-2-glycoprotein were increased. Condusion Idiopathic male infertility was potentially associated with disorder of sperm capacitation and seminal immune function.