蛋白酶体抑制剂MG132对Raji细胞增殖和凋亡的影响

The Effect of MG132,A Proteasome Inhibitor,on Proliferation and Apoptosis of Raji Cells

目的:探讨蛋白酶体抑制剂MG132对人Burkitt's淋巴瘤Raji细胞体外增殖和凋亡的影响。方法:不同浓度(0.5、1.0、5.0、10.0和50.0μmol/L)的MG132处理Raji细胞24 h和48 h,MTT法检测细胞增殖情况;5.0μmol/L MG132处理Raji细胞24 h,流式细胞术进行细胞凋亡率及细胞周期分析。结果:浓度为1.0、5.0、10.0、50.0μmol/L的MG132作用于Raji细胞24 h和48 h后,细胞增殖OD(A_(490nm))值均显著低于对照组(P<0.05),增殖抑制率(20.60±10.28)%～(60.40±2.75)%,随MG132浓度的增加和作用时间的延长细胞增殖抑制率增高,抑制作用呈现剂量和时间依赖性;5.0μmol/L的MG132作用Raji细胞24 h后,实验组细胞凋亡率为25.86%,对照组为0.06%,实验组S期细胞比率(51.70%)较对照组(39.83%)上升,而G0/G1期细胞比率(33.54%)较对照组(46.04%)下降。结论:蛋白酶体抑制剂MG132可显著抑制Raji细胞的增殖并诱导其发生凋亡和G2/M期阻滞。

Objective： To investigate the effect of MG132, a proteasome inhibitor on the proliferation and apoptosis of human Burkitt＇ s lymphoma Raji cells. Methods： Proliferation of Raji cells treated with different concentrations of MG132 （0.5, 1.0, 5.0, 10.0, and 50.0 μmol/L） for 24 h and 48 h were detected by MTT assay. Apoptosis and cell cycle status of Raji cells treated with 5.0 μmol/L MG132 for 24 h were analyzed by flow cytometry. Results：Compared with the control, the proliferation OD（A490nm） value of Raji cells treated with 1.0, 5.0, 10.0, and 50.0 μmol/L MG132 for 24 h and 48 h were significantly decreased（ P 〈 0.05 ） , with inhibition rates ranging from （20.60 ± 10. 28）% to （60.40 ±2.75）%. The inhibitory effect of MG132 on Raji cell proliferation increased concentration and time. After treated with 5.0 μmol/L MG132 for 24 h, the apoptosis rate of Raji cells was 25.86% compared with 0.06% in the controls. More S-phase Raji cells （51.70% vs 39.83% ） and lower rate of G0/G1 cells （33.54% vs 46.04% ） were found in the MG132-treated group. Conclusion： MG132 was demonstrated to inhibit proliferation and induce apoptosis and G2/M arrest of Raji cells.