建立脑出血致应激性溃疡大鼠模型实验的护理配合

Nursing cooperation of establishment a rat model of acute stress ulcer due to cerebral hemorrhage.

目的:总结建立脑出血致应激性溃疡大鼠模型实验的研究效果和护理配合经验。方法:用SD大鼠对照组和实验组各10只进行围手术期护理配合,将实验组大鼠,选择自体尾外侧静脉,用1 ml注射器采血,精确控制200μl血量,准确注入右脑实质内,72 h后处死,观察胃黏膜溃疡发生率,溃疡指数,血清内皮素,血清、脑、胃组织MDA(丙二醛)和SOD(超氧化物歧化酶)测定。对照组大鼠不做颅内手术穿刺注血处理。结果:鼠尾外侧静脉穿刺成功率达100%。实验组胃黏膜溃疡发生率为30%,溃疡指数为15,两组血清、脑、胃黏膜的MDA、SOD,脑神经元细胞、胃壁细胞超微结构电脑图像定量分析比较均有统计学意义(P<0.01);胃壁细胞线粒体体积密度(Vv)有统计学意义(P<0.05);血清内皮素没有统计学意义(P>0.05)。对照组未见任何溃疡病灶。结论:脑注血量200μl致脑出血可有效致使应激性溃疡发生。选择大鼠体尾外侧静脉采血部位,精确控制注入脑部血量,保持饲养环境清洁,合理喂食等护理配合,是确保大鼠脑出血致应激性溃疡模型制作成功的重要环节。

Objective：Summarise experiences of the establishment of cerebral hemorrhage caused by acute stress ulcer rat model of care with study the effect. Methods：Twenty SD rats were randomly divided into control group（n = 10） and experimental group（n = 10） which right intracerebral hemorrhage was formed by inject 200 ul autologous caudal venous blood by 1 ml injector with perioperative nursing in cooperation nursing, the incidence of acute stress ulcer, ulcer index were observed and serum endothelin level, MDA content and SOD activity in serum, intracerebral and gastric mucosa were measured, and the pathological examination of the cerebral tissue and gastric mueosa by light microscopy and electron microsoopy was analysed at 72 hours of the experiment. Control group ,no surgical puncture of intracranial injection of blood treatment. Results： Lateral tail vein puncture success rate of 100%, Experimental group, the incidence of acute stress ulcer was 30% ,ulcer index waslS. Compared with control group, MDA content and SOD activity in serum, intraccrebral and gastric mucosa （P 〈0.01）, Rsv mit in neuron and gastric parietal cell（P〈0.01） ,Vvmit in gastric parietal cell （P 〈0.05）were statistically difference in experiment group, serum endothelin level （ P 〉 0.05 ） were not statistically difference in experiment group. Ulcer lesions were not observed in control group. Conclusions ： Acute stress ulcer by cerebral hemorrhage can be sucessfully induced by injecting 200 p,1 autologous caudal venous blood of rats. Select the tail of rat lateral venous sampling and the precise amount of bleeding control, keeping the breeding of animals the environment clean, reasonable feeding was to ensure that the cerebral hemorrhage due to stress ulcer model making important aspect of success.