用植物提取物筛选与酵母甾醇乙酰化循环相关的物质

Screening for Substances Related to Acetylation/Deacetylation Cycle in S. cerevisiae with Plant Extracts

为了确定酿酒酵母中乙酰/脱乙酰循环是否参与细胞对某些甾体化合物或其类似物的毒性解除过程,将野生型(BY4742)、脱乙酰酶SAY1单突变体(say1Δ)、乙酰基转移酶ATF2单突变体(atf2Δ)和SAY、ATF2双突变体(atf2Δsay1Δ)酵母菌分别培养在含21种不同植物提取物的培养基中,比较其生长状况.结果显示,在含80 mg/mL黄芪提取物的培养基中,细胞生长必须依赖一个完整的乙酰化/脱乙酰循环的作用;而在含20 mg/mL生姜提取物的培养基中,其生长则必须依赖ATF2(乙酰化作用)的缺失.可以推测甾醇乙酰化/脱乙酰循环途径很可能间接参与了某些甾族化合物或其类似物的解毒过程.

To examine whether the sterol acetylation/deacetylation cycle might have a function in the detoxification of cells from potentially toxic steroid or steroid-like compounds, we examined the growth of wild type （BY4742）, deacetylase mutant（say1△ ）, acetyltransferase ATF2 mutant （atf2△） and their double mutant （atf2△say1△）cells in the presence of various plant extracts. This analysis reveals a strong growth advantage of cells fully expressing SAY1 and ATF2 on media containing 80 mg/mL Astragalus root extract, while only cells that lack the capacity to form acetylated sterols can survive on media containing 20 mg/mL Ginger extract. Our data have shown that acetylation/deacetylation cycle might function as an indirect detoxification pathway from some steroid or steroid-like compounds.