219株大肠埃希菌耐药性分析及产ESBLs酶的检测

The antibiotic resistance analysis of 219 strains escherichia coli(E. Coli) and the detection of ESBLs-producing E. coli

目的分析近年来本院临床分离大肠埃希菌的耐药状况以及ESBLs酶和AmpC酶的检出情况，为临床合理使用抗生素提供可靠的实验依据。方法收集本院2004年1月-2008年12月5年间各种临床标本中分离的大肠埃希菌219株，应用API细菌鉴定系统进行鉴定，并用K-B法进行药敏实验，同时，运用CLSI推荐方法检测ESBk酶和AmpC酶。结果大肠埃希菌对泰能、哌拉西林／三唑巴坦、头孢吡肟、头孢他啶敏感，耐药率分别为5.02％、24.66％、21.46％、26.27％，对阿莫西林、氨苄西林、复方新诺明、妥布霉素、氧氟沙星的耐药率均〉70％。结论临床分离大肠埃希菌耐药率和耐药性逐渐增强，临床应结合药敏结果合理使用抗生素，减少其耐药菌株的扩散，产ESBLs和AmpC酶大肠埃希菌的检出应引起临床高度重视。

Objective To investigate the antibiotic resistance of Eseheriehia coli （ E. eoli） and the detection of ESBLs-producing and AmpC-producing E. coli in the first affiliated hospital of chengdu medical college and provide the credible antimi-crobial susceptibility test （ATB） results for clinical antibiotic use. Methods A total of 229 clinical isolates or E. coli were collected from different districts of the first affiliated hospital of chengdu medical college from January 2004 to December 2008. Bacteria were identified with API system. K-B methods were performed to detect the drug resistances profiles of these strains. ESBLs-producing and AmpC-producing E. coli were detected according to CLSI. Results E. coli was sensitive to imipenem, piperacillin tazobactam, cefepime, eeftazidime, and the antibiotic resistance was 5.02%, 24.66%, 21.46% ,26. 27%, respectively. E. coli was resistant to amoxicillin, bactrim, ampicillin, tenebrimycin, Ofloxacin and the antibiotic resistances were all above 70%. Conclusion The antibiotic reisistant of E. coli isolated from clinic was increased. Clinical antibiotic use should accord to the AST results. We must pay more attention to ESBLs-and AmpC-produging E. coli.