应用新鲜冷冻血浆进行TC、TG准确度传递研究

Research of accuracy transfer of cholesterol and triglyceride by fresh mixed refrigerated plasma

目的探讨应用新鲜冷冻血浆作为国产试剂TC、TG校准品的可行性。方法按EP9-A文件要求和NCEP目标计划,以罗氏系统测定的新鲜血清结果为参考,将其准确度传递到以新鲜混合冷冻血浆为校准品的国产甲试剂/日立7170A的临时分析系统上。结果临甲系统测定偏差TC:2.69、1.20、1.00;TG:11.73、4.84、1.90。TC在低、中、高,TG在中、高水平的EA>预期偏差95%可信区间上限。结论通过准确度传递,新鲜混合冷冻血浆可以作为国产血脂试剂的校准品。

Objective To discuss the feasibility of using fresh refrigerated plasma as calibrator of Homemade TC ＆ TG reagents. Methods According to the profile of EP9-A and NCEP traget program, using fresh serum results determined by Roche System as the reference, the accuracy was transferred to Homemade Reagent / Hitachi 7170A Temporary Analysis System which used fresh mixed refrigerated plasma as calibrator. Results In Temporary System I, Determination bias of TC were ：2.69,1.20, 1.00 ,and those of TG were：11.73,4.84,1.90. The EA of low, medium and high levels of TC ,adn the Es of medium and high levels of TG in Temporary System I 〉 upper limit of the 95% confidence interval of expected bias. Conclusion Fresh mixed refrigerated plasma can be used as calibrator of homemade blood lipid reagent through accuracy transfer.