MMP-9在结肠癌中的表达及其临床意义

Expression of MMP-9 in Colon Cancer and its Clinical Significance

[目的]探讨MMP-9在结肠癌中的表达及其在结肠癌进展中的临床意义。[方法]采用Real-Time RT-PCR、免疫组化、计算机图像分析方法定量检测200对结肠癌和正常结肠组织中MMP-9 mRNA和蛋白水平的表达,分析其与临床病理特征的关系。[结果]在结肠癌组织中的MMP-9mRNA表达水平明显高于正常结肠组织(P=0.001),MMP-9 mRNA表达水平与Dukes分期(r=0.98,P=0.000),淋巴结转移(r=0.69,P=0.000)及组织学分化程度(r=-0.724,P=0.015)相关。MMP-9蛋白在结肠癌中的免疫组织化学染色强度(1.96±0.10)高于正常结肠组织(1.21±0.20,P=0.003)。MMP-9蛋白表达水平与Dukes分期(r=0.857,P=0.025)及淋巴结转移(r=0.661,P=0.000)相关。[结论]MMP-9在结肠癌中的表达明显升高,在结肠癌的进展中具有重要作用。

[Purpose] To investigate the expression of matrix metalloproteinase-9 （MMP-9） in colon cancer and its clinical significance. [Methods] Expression of MMP-9 mRNA and protein were detected by quantitative-Real-Time （RT-PCR）, immunohistochemical staining and computerized image analysis in 200 paired colon cancer and normal colonic tissue, MMP-9 expression and clinicopathologic features was analyze. [Results] MMP-9 mRNA expression in colon cancer tissue was significantly higher than that in normal tissue （P=0.001）. It correlated with Dukes＇ staging（r=0.98, P=0.000）, lymph node metastasis（r=0.69,P=0.000） and grading（r=-0.724,P=0.015）. MMP-9 protein expression in colon cancer tissue （1.96±0.10） was higher than that in normal tissue （1.21±0.20, P=0.003）, and was correlated with Dukes＇ staging（r=0.857,P=0.025） and lymph node metastasis（r=0.661,P=0.000）. [Conclusion] MMP-9 expression in colon cancer increases markedly and plays an important role in progression of colon cancer.