静脉内皮细胞的培养及异体血管种植

Cultivation and allogenic vein transplantion of venous endothelial cells

目的探讨血管内皮细胞种植于异体血管的可行性。方法采用胶原酶法消化获取原代内皮细胞。原代细胞经培养后,体外扩增至一定数量后高密度种植于经处理的异体血管上,继续培养至生长牢固。观察细胞状态和生长状况。结果内皮细胞体外培养生长正常,可顺利扩增至需要数量;其分泌von Willebrand因子功能传代后无明显变化,传代变化无统计学差异(P>0.05)。种植后细胞生长正常。结论血管内皮细胞可以经过体外培养扩增,其分泌功能无变化,在异体血管上生长良好。

Objective To study the feasibility of cultivaed venous endothelial cells （ VEC ） transplanting to allogenic vein. Methods Endothelial cells were gained from veins after digestion with 0.2 % collagenase. After VEC was cultivated, they were seeded into allogenic vein, which was lack of endothelium, with high density, and continuitly cultured in endothelial cell medium. The multiplied cells were lined in vitro onto the luminal surface of allogenic vein that was disposed by freeze-drying and radiation. The cultivated cells were confirmed as endothelial cells by yon Willebrand factor. Results Primary endothelial cells were obtained after the in vitro cultivation, and their number was successfully expanded to meet the needs of the experiment. The function to secrete yon willebrand factor showed no significant change （ P 〉 0.05 ）. After allogenic vein transplantation, the cells grew normally. The funtion to excrete yon Willebrand factor was same as before. Conclusions Endothelial cell can grow well in vitro with no change in secretory function, and have exellent growth on allogenic vein.