Fascin1基因表达对胃癌细胞系MKN45增殖与凋亡的影响

The effect of fascin 1 on proliferation and apoptosis in gastric cancer cell line MKN45

目的探讨fascin1基因表达对胃癌细胞系MKN45增殖与凋亡的影响及其意义。方法利用RNA interference(RNAi)技术稳定沉默fascin1基因的表达,Western blotting及RT-PCR检测干扰效率,用MTT比色法和平板克隆形成实验检测转染前后细胞体外增殖能力,用流式细胞术(FCM)及Ho-chest33258荧光染色法检测细胞凋亡的变化。结果在稳定转染fascin1干扰质粒后,胃癌细胞系MKN45的fascin1表达明显下降;与未转染组和空白质粒转染组相比,fascin1干扰质粒转染组的增殖能力明显降低,但细胞凋亡变化不明显。结论fascin1的表达与胃癌细胞系MKN45的增殖有关,而与凋亡无明显相关。

Objective To investigate the effect of fascin 1 on proliferation and apoptosis in gastric cancer cell line MKN45. Methods RNA interference （RNAi） was used to inhibit fascin 1 expression in human gastric cancer cell line MKN45. Expression of fascin 1 was examined by western blotting and RT-PCR. Cell growth ability was evaluated by MTT and clone formation assays. Cell apoptosis was examined by flow cytometry and Hochest 33258 staining method. Results Knockdown of fascinl expression in MKN45 cells resulted in decreased cellular proliferative ability, but not apoptosis. The cloning efficiency of cells transfected with small interfering RNA （ siRNA ） was significantly lower [ （ 36. 5 ± 8. 1 ） % ] as compared to that in MKN45 （ 79 ± 6.3 ） % （ P 〈 0 . 05 ）. Conclusions Expression of fascin 1 is related to the proliferation of gastric cancer cell line MKN45, but has no significant relationship to apoptosis.