摘要
为探讨米糠多糖(RBS)对免疫抑制鸡免疫调节作用的机理,作者采用流式细胞术检测了健康雏鸡、环磷酰胺(CTX)和传染性法氏囊病毒(IBDV)诱导的免疫抑制雏鸡在给予RBS〔剂量150 mg/(kg.d)〕和不给予RBS情况下脾脏和法氏囊细胞的DNA含量、细胞周期及细胞凋亡率。结果显示:(1)CTX+RBS组雏鸡脾脏S期细胞比例和PI值显著高于CTX组(P<0.05),该2组鸡的法氏囊细胞周期没有显著差异(P>0.05)。(2)IBDV+RBS组雏鸡脾脏S期、G2期细胞比例和PI值、法氏囊的S期细胞和PI值均高于IBDV组(P<0.05);(3)CTX+RBS组和IBDV+RBS组雏鸡脾脏和法氏囊细胞的凋亡率分别低于CTX组和IBDV组(P<0.05)。结果表明:RBS能够促进CTX处理鸡脾脏细胞和IBDV感染鸡脾脏和法氏囊细胞DNA复制,对CTX和IBDV诱导鸡的脾脏和法氏囊细胞凋亡过程均有一定的拮抗作用。
To study the immune modulating mechanism of Rice Bran Polysaccharide (RBS) on im- munosuppressed chick, the flow cytometry technique was used to detect the DNA content, cell cycle and apoptosis rate of spleen and bursa cells of chickens in healthy, and immunosuppressed chicks induced by CTX and IBDV under the conditions of giving and not giving with RBS at dos- age of 150 mg/(kg · d) by intragastric administration. The results were as follows: 1) The cell ratio of S phase and proliferation index (PI) in spleen of CTX+RBS group were higher than that of CTX group (P〈 0.05), whereas there was no significant difference of bursa cell cycle between CTX+RBS and CTX group (P〉0.05). 2) The cell ratio of S and G2 phase and the PI in spleen and the cell ratio of S phase and the PI in bursa of IBDV+RBS group were higher than that of IB- DV group (P-Q0.05). 3) The apoptosis rate of spleen and bursa in CTX+RBS and IBDV+RBS group were lower respectively than that of CTX and IBDV group (P〈0.05). These data indicated that the RBS could improve DNA replication of spleen cells in chickens treated with CTX, as well as spleen and bursa cells in chickens artificially infected by IBDV, and that the RBS could in- hibit the apoptosis of spleen and bursa cells in chickens treated with CTX and IBDV.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2009年第6期916-921,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
河北省“十五”科技攻关项目(04220401D)
关键词
米糠多糖
流式细胞术
免疫抑制
细胞周期
细胞凋亡
鸡
rice bran polysaccharide
flow cytometry
immunosuppression
cell cycle
apoptosis
chicken