基因芯片技术检测142例尖锐湿疣HPV基因型的临床分析

Clinical analysis of HybriMax on Human Papillomavirus Genotypes Detection in 142 Cases with Condyloma Acuminate

目的:评价核酸分子快速导流杂交基因芯片技术(HybriMax)检测尖锐湿疣(CA)组织中人乳头瘤病毒(HPV)基因型的临床应用。方法:采用HybriMax技术,对142例尖锐湿疣患者皮损组织进行HPV感染分型检测。结果:在受检的CA患者中21种HPV亚型共检出18种,未发现HPV18、35和56型。142份CA组织标本中HPV阳性137例,总检出率是96.48%,单一基因型别阳性率是58.45%(83/142),多重基因型别阳性率是38.03%(54/142);各基因型别检出率由高到低依次是HPV6(64.08%)、11(34.51%)、52(7.75%)、33(7.04%)、58(7.04%)、16(5.63%)、59(4.23%)、31(3.52%)和68(3.52%)。高危型HPV检出率为42.96%(61/142),高危型HPV多以混合感染形式存在,其多重感染率达到86.89%(53/61),其主要基因型中女性高危HPV型感染率55.56%(20/36)明显高于男性的31.13%(33/106)。结论:尖锐湿疣主要基因型感染是低危型HPV6,高危型HPV感染率高并多以混合感染形式存在。HybriMax技术检测尖锐湿疣患者HPV基因型准确性高,并可快速分型,是临床HPV感染分型检测有效方法。

Objective：To evaluate the application of flow- through hybrization and gene chip （HybriMax） on human papillomavirus （HPV） genotypes detection in condyloma acuminate （CA）. Methods： 142 patients with condyloma acuminate were detected for HPV genotypes by HybriMax. Results：18 genotypes were found in total 21 HPV subtypes, except HPV18, HPV35 and HPV56. 137 HPV positive samples were detected from 142 clinic samples, the total positive rate was 96. 48%, the rate of single type of HPV was 58.45% （83/142）, the positive rate of multiple types of HPV was 48.03% （54/142） ; HPV6（64.08% ） was ranked in first in respective genotypes , then followed by HPV11 （34.51%）, 52（7.75%）, 33（7.04%）, 58（7.04%）, 16 （5.63%）, 59（4. 23% ）, 31 （3.52%） and 68 （3.52%） ; The positive rate of high - risk HPV was 42.96% （61/142）, the infection rate of high - risk HPV genotypes were 86. 89% （53/61） with multiple - genotype. The infection rate of high - risk HPV dominating genotypes in female was 55.56% （20/36） and 31.13% （33/106） in male. Conelusion： The dominating genotype in condyloma acuminate was HPV6 in low - risk HPV, high - risk HPV genotypres had high infection rate and the majority of high -risk HPV genotypes exist with multiple -genotype. HybriMax has high accurate rate in HPV genotypes detection with CA and HPV subtype can quickly classify. It is an effective method to detect HPV genotypes in clinical practice.