养殖池塘底泥脲酶活性与水体NH4^＋-N关系的研究

Studies on the Relationship Between Urease Activity in Sediments and NH_4^+-N in Water in Aquaculture Ponds

以天津市东丽区和西青区养殖池塘为对象,采用对照、沸石、脲酶抑制剂、芽孢杆菌及其组合处理,经过培养后,分析了底泥中脲酶活性与底泥和上覆水中NH4+-N含量之间的关系及不同处理对脲酶活性的影响。试验结果表明:底泥脲酶活性与底泥铵态氮含量以及底泥脲酶活性与上覆水中铵态氮含量随时间的变化规律基本一致,且都达到显著相关关系。底泥和上覆水中NH4+-N含量也呈显著正相关;在养殖水体中加入脲酶抑制剂、沸石及去磷脱氮的芽孢杆菌均能抑制脲酶活性,从而减少上覆水中NH4+-N的含量,并且以加入脲酶抑制剂效果最好,其脲酶活性减少量2.12g·kg-1,上覆水中NH4+-N含量减少6.09mg·L-1。

This study was carried in Dongli District and Xiqing District aquaculture ponds in Tianjin, and the experiment included controls, zeolites, urease inhibitors, bacillus and their combination treatments. After cultivation, the relationship among urease activity in the sediments, the content of NH4＋-N in overlying water and in the sediments, and the influence of the different treatments on urease activity wss analyzed. The study results showed that there was significant correlation between the urease activity and NH4^＋-N content in sediments, the urease activity and NH4^＋-N content in overlying water. The correlation between the content of NH4^＋-N in sediments and in the overlying water was also significantly positive. Adding urease inhibitors,zeolites, removed phosphorus and nitrogen bacillus in aquaculture ponds could inhibit urease activity, further decrease the NH4^＋- N content in overlying water. Among these treatments, urease inhibitors had the best effect. Its highest reductions of urease activity could reach 2.12g·kg^-1 and the highest reductions of NH4^＋-N content in overlying water could reach 6.09g·kg^-1