微卫星分析四个大黄鱼群体的遗传多样性

Genetic diversity of four large yellow croaker(Pseudosciaena Crocea Richardson) populations using microsatellite markers

为保护人工养殖大黄鱼的种质资源,用15对微卫星标记对来自浙江沿海地区的四个大黄鱼养殖群体共171个个体进行了遗传多样性分析。结果显示,这些标记在四个群体中均表现出丰富的多态性;共检测到206个等位基因;各基因座观测等位基因数7～23个,平均等位基因数13.73,大小在88～318bp之间。四个群体的平均观测杂和度(Ho)为0.5981～0.6893,期望杂和度(He)为0.7319～0.7944,多态信息含量(PIC)0.7138～0.7836,表明四个养殖群体在所检测位点均具有较好的多态性。对遗传距离的计算结果表明闵粤东群体与反交群体较近,聚类结果中首先聚到一起。本研究首次选择微卫星标记评估人共选育大黄鱼群体的遗传多样性,并采用高精度的377DNA测序仪进行检测,将对大黄鱼的种质资源保护提供科学依据。

In present study, 15 microsatellite markers were used to analyze the genetic diversity of four cultured stocks （Daiqu, Min-Yue stocks and two groups of their hybrids） of large yellow eroaker （Pseudosciaena Crocea Richardson）. The results showed that all microsatellite markers were polymorphie in four populations. A total of 206 alleles were detected in 171 individuals, and the allele number was ranged from 7 to 23 at 15 loci. The average allele number was 13.73 with the allele sizes of 88 to 318 bp. The value of average observed heterozygosity （Ho） in four populations was 0.7319 to 0.7944, the value of average expected heterozygosity （He） was 0.5981 to 0.6893, and the value of polymorphism information content （PIC） was 0.7138 to 0.7836. The statistics indicated that all tested loci were of a plenty of polymorphisms and abundant genetic diversity in each population. The cluster analysis by UPGMA method showed that Min-Yue stock and MD group were of a close relationship, This analysis of genetic diversity using microsatellite markers could be very useful in studying species conservation for large yellow croaker.