VEGF-C、NF-κBp65及Survivin在鼻咽癌中的表达及临床意义

Expressions of VEGF-C,nuclear factor-kappa B and Survivin in nasopharyngeal carcinoma and their clinical implications

目的通过检测VEGF-C、NF-κBp65及Survivin在鼻咽癌组织(实验组)及鼻咽部慢性炎症组织(对照组)中的表达,探讨它们在鼻咽癌发生、发展过程中的作用及相互关系。方法采用免疫组织化学技术SP法检测VEGF-C、NF-κBp65及Survivin在57例鼻咽癌和20例对照组中的表达情况,并观察其与鼻咽癌的临床病理特征的关系。结果VEGF-C、NF-κBp65及Survivin在鼻咽癌组织的阳性表达率分别为73.68%、61.40%、68.42%,而在对照组中的表达率分别为40.00%、30.00%、20.00%,两者差异有统计学意义(P<0.05)。VEGF-C、NF-κBp65及Survivin的表达与临床分期、淋巴结转移密切相关(P<0.05)。NF-κBp65的表达与VEGF-C及Survivin的表达呈显著正相关,VEGF-C与Survivin的表达亦呈显著正相关。结论NF-κBp65可能通过上调VEGF-C及Survivin的表达促进鼻咽癌的发生、发展及颈部淋巴结转移;VEGF-C和Survivin在鼻咽癌的发生发展中存在着协同作用。

Objective To detect expressions of VEGF-C, NF-κBp65 and Survivin in nasophatyngeal carcinoma（NPC） and chronic nasopharyngitis and to explore their role in the development of NPC as well as their interrelationships. Methods Immunohistochemical （streptavidin-perosidase SP） method was used to analyze protein expressions of VEGF-C, NF-κBp65 and Survivin in paraffin embedded tissues from 57 cases of nasopharyngeal carcinoma and 20 cases of chronic nasopharyngitis and their relation to clinical pathological characteristics of NPC was analyzed. Results Positive rates of VEGF-C, NF-κBp65 and Survivin protein in nasopharyngeal carcinoma tissues were 73.68%（42/57）, 61.40%（35/57）, and 68.42%（39/57）, and in chronic nasopharyngitis tissues were 40.00%, 30.00%, and 20.00%, and the differences were significant between nasopharyngeal carcinoma tissues and chronic nasopharyngitis tissue （P 〈 0.05）. Expressions of VEGF-C, NF-κBp65 and Survivin were closely related to lymph node metastasis and clinical pathological stage（ P 〈 0. 05）. In addition NF-κBp65 protein expression had a positive correlation with beth VEGF-C and Survivin, and expression of VEGF-C was positively correlated with that of Survivin. Conclusion NF-κBp65 contributes to the pathogenesis of nasopharyngeal carcinoma and lymph node metastasis by up-regulating expressions of VEGF-C and Survivin. VEGF-C and Survivin may have synergetic effects on the pathogenesis of nasopharyngeal carcinoma.