摘要
目的评价Sysmex XE-2100血细胞分析仪定量分析外周血幼稚粒细胞(IG)的性能。方法选取202份XE-2100IG报警值>200的样本,分别采用XE-2100和镜检分析IG%,评价两者之间相关性,选取5份不同IG%水平的样本进行XE-2100批内精密度(重复性)评价,分别选取8份标本在4℃和室温进行样本稳定性评价。结果XE-2100分析IG%结果与镜检结果具有良好的相关性(γ=0.8382,P<0.001);5份不同IG%水平样本重复性的变异系数(CV)从4.93%到15.5%;XE-2100分析4℃和室温保存24小时内的样本结果稳定。结论XE-2100定量分析外周血幼稚粒细胞性能良好,基本满足临床实验室要求。
Objective To evaluate the performance of quantitative measurement of immature granulocyte (IG) in peripheral blood using Sysmex XE-2100 hematology analyzer. Methods 202 samples with IG alarm value 〉200 were selected to evaluate the correlation between XE-2100 count and manual morphology count, 5 samples at different levels were selected to evaluate within-run reproducibility for XE-2100 IG count, 8 specimens were selected to evaluate the sample stability at 4℃ and at room temperature. Results The correlativity analysis showed IG agreement between XE-2100 count and the manual morphology count (γ=0.8382, P〈0.001); The within-run reproducibility performed using fresh blood on 5 different level specimens showed a range from 4.93% to 15.5% CV, The percent of IG were stable at both room temperature and after refrigeration for 24 hours. Conclusions The performance of quantitative measurement of immature granulocyte in peripheral blood using Sysmex XE-2100 hematology is well, it can meet the requirements of clinical laboratories.
出处
《实验与检验医学》
CAS
2009年第3期214-216,共3页
Experimental and Laboratory Medicine
关键词
血细胞分析仪
幼稚粒细胞
Hematology analyzer
Immature granulocyte