HPLC测定软肝升白颗粒中黄芩苷的含量

Determination of Scutellarin in Ruanganshengbai Granules by HPLC

目的建立测定软肝升白颗粒中黄芩苷含量的高效液相色谱法。方法采用Diamonsil C18(150mm×4.6mm,5μm)柱分离,以甲醇-水-三乙胺(50∶50∶0.25,用磷酸调节pH2.2)进行洗脱,在277nm处检测。结果黄芩苷在12.04～96.32μg·mL-1范围内浓度与峰面积呈良好的线性关系;黄芩苷的平均回收率为101.1%,RSD为1.41%,n=9。结论本法操作简便,准确度高,重复性好,可作为软肝升白颗粒质量控制方法之一。

Aim To establish an HPLC method for determining scutellarin in Ruanganshengbai Granules. Methods The analysis was performed on Diamonsil C18 （150mm × 4.6mm ,5μm）column with the mobile phase consisting of methanol-water-triethylamine（50： 50：0.25 ,adjusted to pH 2.2 with phosphoric acid）,and the detection wavelength was 277nm. Results The linear calibration curve was obtained within the concentration range of 12.04 96.32 μg.mL^-1. The average recovery of scutellarin was 101.1% ,RSD = 1.41% （n = 9）. Conclusion The method is quick, simple, and reproductive, which can be used to control the quality of Ruanganshengbai Granules.