HPLC法测定五味子浸膏中三种成分的含量

Determination of Three Composition in Compound Chinese Magnoliavine Fruit Extract by HPLC

目的：建立同时测定五味子浸膏中五味予醇甲、五味子甲素与五味子乙素的含量的反向高效液相色谱（RP-HPLC）法。方法：色谱柱为Diamonsil（R）钻石C18柱（200×4．6mm，5um），流动相为甲醇-水（梯度洗脱）。紫外检测波长250nm，柱温30℃，流速1．0ml／min。结果：五味子醇甲、五味子甲素、五味子乙素分别在0．732—5．612ug（r=0．9999）、0．171—1．311ug（r=0．9999）、0．354～2．714ug（r=0．9999）的范围内与峰面积积分值线性关系良好。平均回收率五味子醇甲为99．86％，RSD值为1．76％；五味子甲素为99．53％，RSD值为0.85％；五味子乙素为99．61％。RSD值为0．96％。结论：本法准确，快速，灵敏度高，可用于五味子浸膏中有效成分的定量分析。

Objective： To establish a method for content determination of Schisandrin, Deoxyschizandrin and Schisandrin Bin Chinese magnoliavine fruit extract by RP - HPLC. Methods：The Diamonsil C18 column（200 mm ×4. 6 mm,5 um） was used, mobile phase of water and McOH （gradient elution）was set up, the flow rate was 1.0 ml/min, the column temperature was 30℃ and the detection wavelength was 250nm. Results：The linear ranges of Schisandrin,Deoxyschizandfin and Schisandrin B were within 0. 732 - 5.612ug （ r = 0. 9999） ,0. 171 - 1.311 ug （ r = 0. 9999） and 0. 354 - 2. 714ug （ r = 0. 9999）, respectively. The average recoveries were 99.86% （ RSD 1.76% ） ,99. 53% （ RSD 0. 85% ） and 99. 61% （ RSD 0. 96% ）, respectively. Conclusion：The established method is accurate, reliable and highly specific, and it can be used for the quality contrel of compound Chinese magnoliavine fruit extract.