Rac1、Cdc42单分子探针在活细胞中表达差异的比较

The Comparison of Expression Difference of Rac1,Cdc42 Single Molecule Probe in Living Cells

利用构建的Rac1、Cdc42单分子探针,探索其在NIH3T3、Hela、COS7等活细胞中的最佳表达效果.结果表明在胞浆中表达的探针其荧光值在诱导5～10 min达到最大值,随着时间的推移,其荧光强度逐渐减弱;在细胞膜上表达的探针也是相似的.DNA/转染试剂为1∶3时的表达效果最佳;每毫升转染培养基的质粒DNA含量为4或6μg时,其转染表达可达到最佳;在不同种类的细胞中表达时其差异不明显;在细胞的不同部位表达差异不明显.

The Rac1, Cdc42 single molecule probe were expressed in living cells, such as NIH3T3, Hela, COS7, the best expression of the effect were obtained. In the cytoplasm of the probe in inducing 5 - 10 min to achieve maximum value, with the passage of time, the fluorescence intensity gradually decreased in the membrane of the probe is also similar. DNA/transfection reagents for the expression of 1：3 at best; per milliliter of transfer of the plasmid DNA in the medium to 4 or 6 μg, the transfer can be best expressed in different ceils. The expression of their differences was not obvious in different type of cells. The expression of their differences was not obvious in different parts of cells.