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壳聚糖微球亲和层析提取抑酶肽的研究

Preparation of high purity aprotinin by globate cross-linked chitosan
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摘要 建立了一种高效、实用的抑酶肽制备技术。采用牛肺为原料,利用改性壳聚糖载体,共价偶联胰蛋白酶,亲和层析牛肺提取液,2步制得高比活抑酶肽。抑酶肽抑制比活力为71428 BAEE/mg,酶活性回收率为73.5%。该方法质量稳定,成本较低,吸附剂机械强度高,抗污染能力较强,可反复使用,适宜应用。 The research aimed to establish a kind of effective and practical method to produce aprotinin. The trypsin was covalently linked with chemical modified chitosan and used to purify aprotinin from the extract of cattle lungs by affinity chromatography. The result shows that the specific activity of immobilized trypsin on chitosan is 71 428 BAEE/mg, and the activity recovery of trypsin is 73.5 %. It is accepted as a stable and low-cost method and suitable for purifying aprotinin with high activity in indus'trial manufacturing.
出处 《河北科技大学学报》 CAS 北大核心 2009年第2期92-94,98,共4页 Journal of Hebei University of Science and Technology
关键词 牛肺 亲和层析 抑酶肽 壳聚糖 cattle lung affinity chromatography aprotinin chitosan
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