浙南地区无偿献血者戊型肝炎亚临床感染及病毒血症情况调查

Investigation epidemiology and sub-clinical infection of hepatitis E in voluntary blood donors in south Zhejiang

目的调查浙江南部地区戊型肝炎的感染情况。方法对2008年2—10月间浙江省温州市中心血站的无偿献血者进行整群抽样，检测抗HEVIgG以及IgM抗体，并对抗HEVIgM阳性的标本进行RT-PCR检测，经测序后分析其基因型及序列同源性。结果3044例无偿献血者中，HEVIgG阳性率为33．28％，随着年龄的升高IgG阳性率也有一定的提高，从20岁组的21．16％升高到40岁以上组的50．36％；IgM阳性率0．92％，以31～40岁年龄段感染率最高，为1．90％。通过对IgG和IgM各自阴阳性标本群的分析发现其与献血者的年龄、性别以及血型并无显著相关性。对28例HEVIgM阳性标本进行PCR检测共发现3例核酸阳性，总体阳性率约为10％，其中2例为基因4型，1例为基因1型感染。结论浙南地区的无偿献血者中存在一定比率的HEV病毒携带者。

Objective To investigate the southern region of Zhejiang hepatitis E virus （HEV） infection. Methods A cluster sampling strategy was used to sample all blood donors from February to October in 2008 in Wenzhou blood center. Their blood was tested for IgG and IgM antibody against HEV. Reverse transcriptasepolymerase chain reaction（RT-PCR） and sequencing were applied to detect its genotype and sequence homology in HEV IgM-positive specimen. Results The prevalence of anti-HEV IgG in 3044 cases of blood donors was 33.28%. IgG increased with age. There are certain increase in positive rates between the 20-year-old group and over 40 years of age group from 21.16% to 50.36% . The positive rate of IgM was 0.92% . The ratio of infection among different age group was the highest in the age range from 3ho 40 years and up to 1.90% . IgG and IgM through their negative and positive analysis of samples found in their group with donors age, sex and blood type does not significantly related to each other. Nucleic acids were found in three cases through PCR amplification in all 28 cases of HEV IgM positive samples. The total positive rate was one-thousandth, of which two cases for gene 4, 1 cases of infection for gene 1. Conclusion The results indicate that there was a certain percentage of HEV virus in voluntary blood donors in south Zhejiang.