趋化因子SDF-1α在外周血淋巴细胞中转录与HIV-1感染的相关研究

A transcriptional study of SDF-1α expression in PBL and the correlation between SDF-1α transcription and HIV-1 infection

目的研究趋化因子SDF-1α在HIV感染者外周血淋巴细胞（PBL）中的表达，以及其表达水平与HIV感染的关系。方法研究对象包括97名健康人与238例HIV感染患者，后者包括92例A1～A3阶段无症状患者和146例B1～C3阶段患者。首先分离PBL，提取总RNA并行反转录（RT）-PCR。建立荧光定量PCR方法，分别进行SDF-1α与β-globin定量，根据SDF—1α拷贝数与pglobin拷贝数比值计算R1值。结果在健康人、无症状感染者和艾滋病患者中SDF-1α转录水平不同，HIV-1感染晚期暨艾滋病患者的SDF—1α表达上调。相关分析显示R1值与CD4’T淋巴细胞计数呈明显负相关（P=0．002），而与病毒载量呈明显正相关（P=0．001），表明SDF-1α转录水平与病情进展有一定关系。结论HIV感染晚期患者PBL内SDF-1α转录上调，影响SDF—1α转录上调的机制及SDF-1α表达升高对机体的病理作用值得深入研究。

Objective To determine the transcription of SDF-1α in peripheral blood lymphocytes （PBL） and analysis the correlation between SDF-1α transcription and HIV infection. Methods Three groups of study subjects were recruited： （1） 97 HIV negative healthy donors, （2） 92 HIV patients of Al to A3 stages and （3） 146 HIV patients of B1 to C3 stages. Total RNA was extracted from PBL. Reverse transcription （RT）-PCR and quantification PCR were developed for the SDF-1α transcriptional study. R1 value was calculated based on the ratio of SDF-1α copies to β-globin copies. Results SDF-1α transcription is heterogeneous among the three study groups. The SDF-1α transcription was significantly up-regulated during late stage of HIV infection than the healthy donors. Correlation analysis indicated that RI value was negatively correlated to CD4^＋ T cells counts （P = 0.002） ;and positively correlated to virus load （ P = 0.001 ）. The result demonstrated an association between SDF-1α transcription and disease progression. Conclusion SDF-1α transcription was significantly up-regulated during late stage of HIV infection. It would be worthwhile to determine the mechnism of HIV affecting on SDF-1α genes transcription and the up-regulated SDF-1α expression on the disease progression.