诺如病毒酶联免疫检测试剂盒效果的研究

Study on effect of enzyme linked immunasorbent assay kits of norovirus

目的比较RIDASCREEN Nomvims 3^rd Generation kit（R—biopham AG，darmstadt，Germany）（必发试剂盒）与IDEIA NLV kit（DAKOCytomation．，Ely，UK）（dako试剂盒）两试剂盒检测诺如病毒效果。方法用此两种试剂盒以及RT-PCR方法对长春和广州两地的308份腹泻患儿粪便标本各检测一遍。对RT-PCR阳性标本进行基因测序、序列对比以确定型别。结果以RT-PCR方法为金标准，必发试剂盒的灵敏度和特异度分别是96．10％和93．51％，阳性预测值和阴性预测值是83．14％和98．63％；dako试剂盒的灵敏度和特异度分别是95．83％和95．76％，阳性预测值和阴性预测值是87．34％和98．69％。结论必发试剂盒因为具有满意的灵敏度和较好的特异度，应该成为ELISA检测的首选。

Objective Effects of RIDASCREEN Norovirus （ C 1401 ） 3rd Generation kit （ R-biopham AG, darmstadt, Germany）and IDEIA NLV kit （DAKOCytomation., Ely, UK）were compared for detecting human norovirus（HuNV） in fecal sample. Methods The performance of the ELISA was compared with that of the reverse transcription polymerase chain reaction （RT-PCR） by testing a panel of 308 fecal samples collected from patients involved in outbreaks of gastroenteritis in Chang Chun and Guang Zhou. Gene sequencing was performed to positive samples tested by RT-PCR to determine genotype compared with standard sequences. Results RT-PCR is gold standard, RIDASCREEN Norovirus （C 1401 ）3^rd Generation kit had a high sensitivity of 96.10% but a specificity of 93.51%, and Dako kit had a low sensitivity of 95.83% but a high specificity of 95.76% . Conclusion RIDASCREEN Norovirus（ C 1401 ）34 Generation kit is more Satisfactory for a preliminary screening.