摘要
目的观察罗格列酮对兔髂动脉球囊内膜剥脱术后,脂联素表达水平及内膜增殖的影响,并探讨罗格列酮预防血管再狭窄的可能机制。方法将30只雄性新西兰大白兔随机分为3组,即正常对照组(给予普通饮食)、模型组(给予高脂饮食+球囊剥脱术)和罗格列酮组(给予高脂饮食+球囊剥脱术+罗格列酮)。分别于球囊损伤术后第1、4周,取血测定血脂、血糖;用ELISA法测定血浆脂联素的水平。取髂动脉标本进行病理形态学观察及弹力纤维染色。用免疫组化染色法测定增殖细胞核抗原(PCNA)的表达。结果成功建立了兔髂动脉血管内皮损伤模型。与模型组兔相比,罗格列酮组在术后第1、4周末,总胆固醇(TC)、三酰甘油(TG)和低密度脂蛋白-胆固醇(LDL-C)等指标均明显降低(P<0.01),HE及弹力纤维染色提示,内膜增殖程度显著减轻,PCNA的表达减少,血浆脂联素的水平明显升高(P<0.05或P<0.01)。以上变化在术后4周末更为显著。结论罗格列酮可有效抑制血管损伤后内膜的增殖,其机制可能与通过PPARγ途径增加脂联素的表达水平有关。
AIM ： To study the effects of rosiglitazone on the expression of adiponectin and intimal proliferation after balloon endothelial denudation in rabbits and to explore the mechanism of rosiglitazone in preventing restenosis. METHODS： Thirty male New Zealand white rabbits were randomly divided into control group （normal diet）, model group （hypercholesterol diet plus balloon endothelial denudation） and rosiglitazone group （balloon endothelial denudation plus hypercholesterol diet supplemented with rosiglitazone）. Serum lipids and adiponectin levels were measured at 1 week and 4 weeks after balloon endothelial injury. Local iliac arteries were sectioned for the analysis of pathological morphology and were stained for PCNA immunohistochemistrical analysis. RESULTS： Hypercholesterolemia was successfully induced. After rosiglitazone intervention, significant difference was found in the levels of serum TC, TG and LDL-C between model and rosiglitazone groups （ P 〈 0. 01 ）. Compared with those in the model group, expression of PCNA in rosiglitazone group was significantly lowered and expression of adiponectin significantly increased （P 〈 0.05 ）. These changes were more significant by the end of the 4th week after balloon endothelial denudation. CONCLUSION： Rosiglitazone efficiently inhibits intimal proliferation during restenosis after balloon injury, which may be related to the increasing expression of adiponectin.
出处
《心脏杂志》
CAS
2009年第4期462-466,共5页
Chinese Heart Journal
