Id3、VEGF、C-erbB-2在乳腺癌中的表达及临床意义

Expression of Id3,VEGF and C-erbB-2 and clinical significance in patients with breast cancer

目的研究分化/DNA结合抑制因子3(Id3)、血管内皮生长因子(VEGF)和人类表皮生长因子受体2(HER-2/C-erbB-2)的表达与乳腺癌患者临床病理因素之间的相关性。方法采用免疫组化SABC法分别检测70例乳腺癌、40例乳腺癌旁组织、20例乳腺良性肿瘤(纤维腺瘤)和20例正常乳腺组织中Id3、VEGF和C-erbB-2蛋白的表达。结果Id3、VEGF、C-erbB-2在乳腺癌组织中的阳性表达率(分别为88.6%、95.7%、20.0%)均明显高于其他三种组织水平并进行统计学分析(P<0.01)。Id3、VEGF、C-erbB-2蛋白在不同年龄、淋巴结转移情况、肿瘤大小、组织学分级的乳腺癌患者中的表达无统计学差异(P>0.05),但C-erbB-2蛋白在不同pTNM分期的乳腺癌患者中表达差异显著(P<0.05)。Spearman等级相关分析表明,Id3与VEGF的表达呈正相关(r=0.367,P=0.002),C-erbB-2与雌激素受体、孕激素受体的表达呈负相关(r=-0.335,P=0.005;r=-0.300,P=0.012)。结论Id3、VEGF和C-erbB-2蛋白在乳腺癌患者中高表达。C-erbB-2可作为判断乳腺癌预后的一个重要参考指标,其与乳腺癌患者的雌、孕激素受体的表达呈负相关;Id3的表达与VEGF的表达呈正相关。

Objective To investigate the correlation between clinicopathological parameters and the expressions of inhibitor of differentiation/DNA binding 3 （Id3）, vascular endothelial growth factor （VEGF） and human epidermal growth factor receptor-2 （HER-2/CerbB-2） in patients with breast cancer. Methods The protein expressions of Id3, VEGF and C-erbB-2 were detected by SABC immunohistochemistry in 70 spceimens of breast cancer, 40 specimens of tissue adjacent to breast cancer, 20 specimens of fibroadenoma and 20 specimens of normal breast tissue. Results The expressions of Id3, VEGF and C-erbB-2 in breast cancer tissue （88. 6%, 95. 7% and 20. 0%, respectively） were significantly higher than that in the other tissues （P〈0. 01）. No statistical correlation existed between the expressions of Id3, VEGF and C-erbB-2 among the patients as grouped by age, lymph nodes metastasis, tumor size and histological grading （P〉 0. 05）, while the expression of C-erbB-2 protein substantially varied among the patients of breast cancer in different pTNM stages （ P〈 0. 05）. Spearman rank correlation analysis revealed that the expression of Id3 was positively correlated with that of VEGF （r=0. 367, P= 0. 002）, whereas the expression of C-erbB-2 was negatively correlated with that of estrogen receptor and progesterone receptor （r= -0. 335, P=0. 005 and r=- 0. 300, P= 0. 012, respectively）. Conclusions Over-expressions of Id3, VEGF and C-erbB-2 exist in the patients with breast cancer. C-erhB-2, which is negatively correlated with the expressions of estrogen receptor and progesterone receptor, may serve as an important reference for prognosis of breast cancer. The expression of Id3 is positively correlated with that of VEGF.