实验性脉络膜新生血管的定量评价

Quantitative estimation of experimental choroidal neovascularization

目的评价氪激光诱导下大鼠脉络膜新生血管(choroidal neovascularization,CNV)面积计算的可行性。方法采用氪激光击破大鼠右眼Bruch膜的方法诱导24只大鼠产生实验性CNV,另一眼作正常对照眼。眼底彩照及眼底血管荧光造影检查(fundus fluorescein angiography,FFA)分别于光凝后1周、2周、3周、4周时观察大鼠眼底CNV渗漏情况,脉络膜铺片技术测量CNV面积。结果氪激光大鼠CNV模型成模率3周、4周时稳定(75.00%、78.75%)。从FFA、脉络膜血管铺片技术可见大鼠CNV面积随造模后时间延长呈明显递增生长(P<0.05),3周、4周时差异不明显,病理切片亦可见CNV中新生血管数量的增多。结论FFA检查、脉络膜血管铺片技术、病理切片均可作为评价CNV生长的指标,其中脉络膜血管铺片技术是CNV定量评价的可靠方法。

Objective To investigate the feasibility of calculating the areas of krypton laser-induced choroidal neovascularization （CNV） in rats. Methods Right eyes of 24 rats received krypton laser irradiation to break through Bruch membrane for the establishment of experimental CNV, and the left eyes were as controls. CNV leakage at ocular fundus was observed with fundus photography and fundns fluorescein angiography （FFA） at 1 week,2 weeks,3 weeks and 4 weeks,respectively. CNV areas were measured with stretched preparation of choroid. Results The rates of succeed laser- induced CNV model became stable at 3 weeks and 4 weeks （75.00% ,78.75% ）. CNV areas increased significantly with time prolonged after CNV model established by FFA and stretched preparation of choroid （P 〈 0.05 ）. The differences were not significant at 3 weeks and 4 weeks. The increase of CNV was also found at pathological sections. Conclusions FFA, stretched preparation of choroid and pathological sections can be used as index for estimating CNV,and stretched preparation of choroid for quantitative analysis of CNV is reliable.