根癌农杆菌介导的甜瓜遗传转化

Agrobacterum tumefaciens-mediated genetic transformation in melon

以甜瓜子叶为根癌农杆菌介导转化的受体,通过GUS基因瞬间表达率的分析,研究影响转化体系的重要试验参数。试验结果表明,甜瓜对卡那霉素耐性高,筛选浓度可高达150 mg/L,Ag+能促进转化率,而AS不能促进转化率。前期的消毒效果与季节有很大的关系,不同季节选择不同的消毒方式更能节约种质资源。

The experimental parameters were investigated in Agrobacteraum-mediated transformation of melon cotyledons based on the transient expression rate of GUS gene. The result showed that melon cotyledons had high tolerance to Kanamycin with concentration of 150 mg/L, which was used to screen the cotyledon of melon after transformation. Addition of Ag^＋ into cocultivation medium was more beneficial for improving transformation frequency, however, acetosyringone （AS） was useless. The different disinfection methods should be taken according to different seasons for saving the melon seeds.