彩色马蹄莲组培快繁体系优化

Optimized rapid propagation system in tissue culture of Zantedeschia hybrida

用0.1%的HgCl2对彩色马蹄莲外植体进行常规灭菌,一年生种球的块茎芽灭菌时间12 min对于提高初代培养成活率的效果最好,二年生和三年生种球的块茎芽的最佳灭菌时间以16 min效果最好;在MS培养基上,BA 2.0+GA3 0.3激素配比组合可以比较稳定地诱导出较高的5.3倍的增殖倍数,且不定芽生长良好,是最佳激素和浓度配比;采用1/2 MS培养基,IBA浓度为0.5 mg/L,蔗糖浓度为10 g/L时,壮苗生根的效果好;室外炼苗3 d有利于彩色马蹄莲组培苗瓶苗移栽成活,80 d后组培苗成活率达88%,试验过程中组培苗瓶苗在3月10日移栽成活率最高,达到100%。

The explants of Zantedeschia hybrida were disinfected by 0.1% HgCl2. The results showed that 12 rain was the best sterilization time for annual tuber bud while 16 rain was the best sterilization time for biennial and three-year-old tuber bud. The.best hormone combination was BA 2.0 mg/L ＋ GA3 0.3 mg/L for MS medium, which could induce a higher proliferation multiple of adventitious buds of 5.3. Meanwhile, the adventitious buds growed well. The suitable growing medium for plantlet roofing was 1/2 MS medium ＋ 0.5 mg/L IBA ＋ 10 g/L sucrose. It was benefit to increase the transplantation survival rate when the plantlets were treated with 3 d outdoor acclimation. When transplanted after 80 d, the survival rate of plantlets was 88%. In this experiment, March 10 was the best transplanting date, on which the survival rate of plantlets achieved 100%.